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The impact of MRSA on macrophage infiltration and polarization in mouse lung tissues
1
Anhui Medical University Children's Medical Center，Fifth Clinical Medical College. Anhui Hefei 230031,China
2
Anhui Children's Hospital Emergency Department. Anhui Hefei 230041,China
*
Author to whom correspondence should be addressed.
JPHE 2024 7(1):246; https://doi.org/10.69610/j.phe.20240712
Received: 1 May 2024 / Accepted: 11 July 2024 / Published Online: 12 July 2024
Abstract
Objective: To establish a model of methicillin-resistant Staphylococcus aureus（MRSA） pneumonia and observe the effect of MRSA on macrophage polarization. Methods: The MRSA pneumonia model is established by adding 1×108CFU/mL MRSA bacterial solution to BALB/c mice through nasal drip. To make pathological specimens of lung tissue and observe the inflammation of the lungs;to detect the expression of F4/80, CD11B, CD206, and CD86 in mouse lung tissue through flow cytometry to reflect the polarization of macrophages and their M1/M2.
Result: Compared with the uninfected group, macrophages have increased significantly in infected group; The expression of CD206 in macrophages has significantly enhanced after infection, indicating the macrophage polarization towards M2;
Conclusion: MRSA pulmonary infection leads to a significant infiltration of macrophages in lung tissue and their polarization towards the M2 phenotype.
Keywords:
Methicillin resistant staphylococcus aureus;
Pneumonia;
Macrophage polarization;
M2 macrophage;
Copyright: © 2024 by Chen, Xu and Tian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (Creative Commons Attribution 4.0 International License). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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ACS Style
Chen, M.; Xu, W.; Tian, K. The impact of MRSA on macrophage infiltration and polarization in mouse lung tissues. Journal of Public Health & Environment, 2024, 7, 246. doi:10.69610/j.phe.20240712
AMA Style
Chen M, Xu W, Tian K. The impact of MRSA on macrophage infiltration and polarization in mouse lung tissues. Journal of Public Health & Environment; 2024, 7(1):246. doi:10.69610/j.phe.20240712
Chicago/Turabian Style
Chen, Mingxiao; Xu, Weihua; Tian, Keyin 2024. "The impact of MRSA on macrophage infiltration and polarization in mouse lung tissues" Journal of Public Health & Environment 7, no.1:246. doi:10.69610/j.phe.20240712
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